Plant surfaces as vehicles of Bacillus cereus responsible of human food poisoning

• Introduction A major concern in food safety is the contamination of fresh and stored food with spoiling bacteria that provoke human poisoning. Bacillus cereus is a common food-borne pathogen responsible of important poisoning outbreaks and severe bacteraemia and septicaemia. Poisoning caused by B. cereus is classified in two main categories: emetic and diarrheic. The emetic poisoning is correlated to the production of cereulide. This toxin is very heat stable, and it can be produced in the food contaminated by B. cereus cells. Diarrheic poisoning is provoked by the enterotoxin hemolysin BL, the non-hemolytic enterotoxin and the cytotoxin K. • Objective To study the interaction of B. cereus with plants as a bacteria reservoir, and in ready-to-eat fruits and vegetables. • Materials & Methods A collection of strains implicated in food-borne outbreaks were tested in vitro for a battery of phenotypes related to bacterial multicellular behaviour and thus interaction with host. 1. Solid or liquid media were used to study biofilm formation, motility or adhesion to surfaces. 2. Leaves, fruits and vegetables (melon leaf, cucumber leaf and fruit and endive) were used to study the persistence of B. cereus over time and their distribution and organization by electron microscopy. • Results All the strains behaved similarly in vitro, only some persisted on plant surfaces. Among them, the emetic strain AH187 was selected because bacterial cells persisted on a concentration of 104-105 CFU per gram of leaf, vegetable or fruit, with a sporulation rate of 40%. The electron microscopy images showed the organization of bacteria in well-developed biofilms with visible extracellular matrix. Finally, mass spectrometry analysis proved the presence of some isoforms of cereluide on the different surfaces. • Conclusion The fact that cells of B. cereus persist in leaf surface mainly as vegetative cells are indicative of their ability to adapt to the physico-chemical changeable phyllosphere, and thus to produce the emetic toxin cereulide. The presence of spores, and the formation of biofilms can be indicative of the versatile adhesive properties of this strain to diverse surfaces. Altogether are supportive of the importance of plant surfaces either as reservoir of bacterial cells or as vehicles for further contamination and food poisoning.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

Planck/SDSS Cluster Mass and Gas Scaling Relations for a Volume-Complete redMaPPer Sample

Using Planck satellite data, we construct SZ gas pressure profiles for a large, volume-complete sample of optically selected clusters. We have defined a sample of over 8,000 redMaPPer clusters from the Sloan Digital Sky Survey (SDSS), within the volume-complete redshift region 0.100 < z < 0.325, for which we construct Sunyaev-Zel'dovich (SZ) effect maps by stacking Planck data over the full range of richness. Dividing the sample into richness bins we simultaneously solve for the mean cluster mass in each bin together with the corresponding radial pressure profile parameters, employing an MCMC analysis. These profiles are well detected over a much wider range of cluster mass and radius than previous work, showing a clear trend towards larger break radius with increasing cluster mass. Our SZ-based masses fall ~24% below the mass-richness relations from weak lensing, in a similar fashion as the "hydrostatic bias" related with X-ray derived masses. We correct for this bias to derive an optimal mass-richness relation finding a slope 1.22 +/- 0.04 and a pivot mass log(M_500/M_0)= 14.432 +/- 0.041, evaluated at a richness lambda=60. Finally, we derive a tight Y_500-M_500 relation over a wide range of cluster mass, with a power law slope equal to 1.72 +/- 0.07, that agrees well with the independent slope obtained by the Planck team with an SZ-selected cluster sample, but extends to lower masses with higher precision.Comment: 13 pages, 7 figure

Planck/SDSS Cluster Mass and Gas Scaling Relations for a Volume-Complete redMaPPer Sample

Using Planck satellite data, we construct SZ gas pressure profiles for a large, volume-complete sample of optically selected clusters. We have defined a sample of over 8,000 redMaPPer clusters from the Sloan Digital Sky Survey (SDSS), within the volume-complete redshift region 0.100 < z < 0.325, for which we construct Sunyaev-Zel'dovich (SZ) effect maps by stacking Planck data over the full range of richness. Dividing the sample into richness bins we simultaneously solve for the mean cluster mass in each bin together with the corresponding radial pressure profile parameters, employing an MCMC analysis. These profiles are well detected over a much wider range of cluster mass and radius than previous work, showing a clear trend towards larger break radius with increasing cluster mass. Our SZ-based masses fall ~24% below the mass-richness relations from weak lensing, in a similar fashion as the "hydrostatic bias" related with X-ray derived masses. We correct for this bias to derive an optimal mass-richness relation finding a slope 1.22 +/- 0.04 and a pivot mass log(M_500/M_0)= 14.432 +/- 0.041, evaluated at a richness lambda=60. Finally, we derive a tight Y_500-M_500 relation over a wide range of cluster mass, with a power law slope equal to 1.72 +/- 0.07, that agrees well with the independent slope obtained by the Planck team with an SZ-selected cluster sample, but extends to lower masses with higher precision.Comment: 13 pages, 7 figure

The Upper Bound Theorem in forging processes: Model of Triangular Rigid Zones on parts with horizontal symmetry

The analysis to determine the necessary forces with which to achieve a plastic deformation in metallic materials, in particular, in forging processes and under conditions of plain strain, has been raised over the years through a double approach; on the one hand, by analytical methods that involve a great complexity in their developments but that allow a direct understanding of the parameters that direct these processes. On the other hand, numerical methods, in which, thanks to the enormous development of computer technology, they provide solutions with a high approximation but, in most cases, do not allow to interpret independently the effect of each one of the parameters that come into play. The development of computers relegated analytical methods to the background. An alternative of great interest to apply these methods comes from the study of the Upper Bound Theorem by means of the Triangular Rigid Zones (TRZ) Model. One of the main limitations in the application of this model come from the fact that it is necessary to define a kinematically admissible velocity field and for complex geometric configurations of parts, this field becomes increasingly complicated. A new approach has delimited, from a theoretical perspective, a modular configuration based on a General Module formed by three TRZ that adapts to any geometry of flat surfaces of the part. Another limitation of the Upper Bound Method is the consideration of the plain strain represented by a flat section with double symmetry. Obviously, this imposition only allows to study a limited number of part configurations, which restricts its application in forging processes since the great majority of forged parts do not present geometrically this double symmetry. The present work releases one of these boundary conditions allowing to expand the possibilities of application of this method.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tec

Compounds produced by two robust Bacillus amyloliquefaciens biocontrol strains involved in antimicrobial activity and plant-growth promotion

Several members of the Bacillus genus are potential candidates to be used as biological control agents to combat pests or plant diseases. The bacterial attributes associated to Bacillus behaviour are mainly: the production of antimicrobial compounds, the plant-growth promotion capability and the induction of systemic resistance in plant host. In previous works, we have demonstrated this multifaceted biocontrol activity of B. amyloliquefaciens CECT8237 (UMAF6639) and CECT8238 (UMAF6614) strains, which contributes to plant protection against bacterial and fungal pathogens. In order to identify the bacterial features responsible for the outstanding biocontrol activity of these strains, their genomes were sequenced and analysed. Firstly, those features previously described for other Bacillus to be involved in the biocontrol activity were localized: i) Biosynthetic genes of secondary metabolites. Apart from the lipopeptides, formerly detected, we have demonstrated the production of other additional compounds that might participate in the antibiosis activity; ii) Biosynthetic genes of the volatile compounds 2,3-butanediol and acetoin, both involved in the induction of plant defence responses. Secondly, genetic singularities non-conserved within the Bacillus genus, which might contribute to the biocontrol ability of B. amyloliquefaciens CECT8237 and CECT8238 were identified in both genomes. Among them, we highlight two genomic regions hypothetically implicated in the production of non-characterized secondary metabolites. Ongoing studies are focused on elucidating the functionality of these uncharacterized regions, leading to a better understanding of the mechanisms of action involved in the robust biocontrol skills of these strains.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

The expression profile of secondary metabolites in biofilms of B. amyloliquefaciens CECT 8237 biocontrol strain

The contribution of Bacillus amyloliquefaciens CECT 8237 (UMAF6639) strain to the plant protection against bacterial and fungal pathogens is mainly based on: i) the production of antimicrobial compounds, ii) the plant-growth promotion capability and iii) the induction of systemic resistance in plant host. In previous works, we demonstrated the relevant implication of the three families of lipopeptides in the biocontrol activity and biofilm formation on melon leaves. The analysis of the genome sequence revealed features previously identified in other Bacillus strains, such as genes related to biofilm formation, phytostimulation and induction of systemic resistance in the host plant, and novel genomic regions non-conserved within the Bacillus genus, and therefore with potential genes implicated in the biocontrol activity. Considering the relevance of biofilm formation and production of secondary metabolites in biocontrol, we analyzed the expression profile of several secondary metabolites produced by CECT 8237 in biofilm inducing conditions. To do so, we optimized an in situ detection method based on MALDI-TOF analysis of secondary metabolites within the bacterial colony and in supernatants and pellicles of B. amyloliquefaciens biofilms. We found a major accumulation of these secondary metabolites in the core and middle area of the colony and in the spent medium compared to pellicle. Further studies will help elucidating the real implication of these molecules in the bacterial ecology or in its mechanisms of defence, against competitors, and/or offense, against pathogens and its possible relation with the niche they occupy. This work was supported by grants from the Plan Nacional I+D+I (AGL-2012-31968) and Incentivos a Proyectos de Excelencia de la Junta de Andalucía (P10-AGR-57-97), both cofinanced by FEDER funds (EU). This work was also supported by grant from Koppert B.V. (8.06/60.4086) and European Research Council-Starting Grant BacBio ERC637971.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

Election of Water Resources Management Entity Using a Multi-Criteria Decision (MCD) Method in Salta Province (Argentine)

At present, the water resources are a strategic element each time more necessary and limited becoming a source of conflicts. For that, it is fundamental to create an independent and competent entity with good reputation and social acceptation. This entity must be able to obtain, store and process all data dispersed in different entities creating a network for these purposes. Finally, it must be able to organize different branches between the government and the final users. Using one of the wellknown Multicriteria Decision Methods(MCDM) with several realistic alternatives and several criteria identified in expert seminars in Salta and Madrid, we have obtained hopeful results and more recently new modifications introduced have generated better results